PD-L1 and MHC-I expression in 19 human tumor cell lines and modulation by interferon-gamma treatment
نویسندگان
چکیده
Methods Nineteen human tumor cell lines were cultured according with ATCC guidelines: 5 colon (Caco-2, SW620, SW480, Colo-205 and HT-29), 4 ovarian (OV-17, OVCAR-3, ES-2, SKOV-3), 3 breast (MDA-MB-231, MCF-7, ZR-75), 3 lung (H441, H1703, H460), 2 prostate (LnCap and PC-3), and 2 pancreatic (CFPAC-1 and ASPC-1). Cells were analyzed by flow-cytometry for PD-L1 (clone 29E.2A3) and MHC-I expression. The surface expression of PD-L1 was considered as low, medium, or high based on the percentage of positive cells (80%, respectively). Cells were also analyzed for PD-L1 mRNA expression by RT-PCR. Experiments were performed with or without IFN-g pre-treatment (10 ng/ml, 24 hours).
منابع مشابه
SUSCEPTIBILITY OF HUMAN WM MELANOMA CELL LINES TO NK AND LAK CYTOTOXICITY AND THEIR RELEVANCE TO THE LEVEL OF MHC CLASS I AND ICAM-l ANTIGEN EXPRESSION
The effect of natural killer (NK) cells and lymphokine activated killer ( LAK) cells was studied on a group of human melanoma cell lines. Peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhI L-2 for producing LAK cells. The cytotoxicity of effector cells was quantified using a 4 hour SI determining the density of antigen expression on tumor...
متن کاملExpression Pattern of Interferon-γ in Human Leukemic T Cell Lines Following Treatment with Phytoheamagglutinin, phorbol myristate acetate and Lipopolysaccharide
Background: As a T helper type 1 (Th1) derived cytokine, Interferon gamma (IFN-γ) is an important regulator of inflammatory immune responses. Furthermore, IFN-γ plays an essential role in defense against tumors and intracellular pathogens. This study was designed to assess the pattern of IFN-γ production in human leukemic (Jurkat and Molt-4) T cell lines in vitro. Methods: Ju...
متن کاملThe effect of bone marrow-derived mesenchymal stem cells to induce PD-L1 molecule on splenic lymphocytes
Background: Mesenchymal stem cells are non-hematopoietic stromal cells that are used in the treatment of many chronic and autoimmune diseases by modulating the immune system. Due to the limitations of using autologous mesenchymal stem cells, the use of allogeneic stem cells is a promising therapeutic approach in the treatment of immunological disorders. This study aimed to investigate the abili...
متن کاملPD-L1/PD-1 expression and tumor-infiltrating lymphocytes in conjunctival melanoma
Conjunctival melanoma (CM) is an infrequent but potentially lethal malignancy, with limited therapeutic options for metastases. Recent inhibitors of the interaction of programmed cell death protein 1 (PD-1) and its ligand PD-L1 are associated with good clinical responses in many malignancies. To investigate the therapeutic potential of targeting the PD-1/PD-L1 axis in CM, we analyzed the expres...
متن کاملProducing Soluble Human Programmed Cell Death Protein-1: A Natural supporter for CD4+T cell Cytotoxicity and Tumor Cells Apoptosis
Background: Programmed cell death protein-1 (PD-1)/PD-L1 pathway is one of the immune checkpoint pathways involved in regulation of the immune responses and suppression of anti-tumor defense. PD-1/B7-H1-blocking antibodies improve immune responses such as cytotoxic activity of CD8+/CD4+T cells and also increase mortality of tumor cells; however their use is accompanied by adverse effects in pat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 2 شماره
صفحات -
تاریخ انتشار 2014